Chainy, an universal tool for standardized relative quantification in real-time PCR.

Mallona I, Díez-Villanueva A, Martín B, Peinado MA.  Chainy, an universal tool for standardized relative quantification in real-time PCR. Bioinformatics. 2017 May 1;33(9):1411-1413. doi: 10.1093/bioinformatics/btw839.

Abstract

Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis.

AVAILABILITY:

Chainy is open source and freely available at http://maplab.cat/chainy.

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